Immunohistochemistry Resource Guide

Table of Contents

Introduction

Immunohistochemistry Workflow

Immunohistochemistry Selection Guide

Pioneering in IHC Technology

Choosing a Detection System

Avidin-Biotin Complex (ABC)-Based Detection

VECTASTAIN^® ABC Systems

VECTASTAIN^® ABC Kits

Choosing a VECTASTAIN^® ABC Kit

> Customizing your VECTASTAIN^® ABC Kit

Polymer-Based Detection

ImmPRESS^® One-Step Polymer Systems

> ImmPRESS^® Two-Step Amplified Polymer Systems

> Choosing an ImmPRESS^® Polymer Kit

> Multiple Antigen Labeling Simplified

Species on Species Detection

M.O.M.^® (Mouse on Mouse) Immunodetection Kits

Choosing an Enzyme Substrate

Enzyme Substrates

> Enzyme Substrate Properties

Multiple Antigen Labeling

Enzyme Substrate Combinations

Counterstaining

> Counterstain/Substrate Compatibility Table

Blocking Background Signal

Secondary and Tertiary Detection Reagents

Mounting Media

Accessory Reagents

ImmPRESS Excel Amplified Polymer Staining Systems

ImmPRESS Excel Amplified Peroxidase (HRP) Polymer Staining Systems are complete staining kits that capitalize on all the advantages of the ImmPRESS HRP Polymer System technology and offer additional sensitivity and convenience.

This system employs a ready-to-use (R.T.U.) Amplifier Antibody, followed by an ImmPRESS Excel HRP Polymer Reagent. These reagents are affinity-purified and extensively cross-adsorbed to ensure high sensitivity and low background. The included ImmPACT^® DAB EqV (equal volume) substrate produces a crisp, dark brown reaction product with excellent sensitivity that is characteristic of the ImmPRESS/ImmPACT combination.

The ImmPRESS Excel Amplified HRP Kits are recommended for applications that require detection of weakly-expressed antigens, in cases of unknown expression levels such as gene knock-in studies, or in determining up-regulation of a given target. This straightforward kit format yields reliable, consistent results and saves time in trying to establish optimal titrations with concentrated detection reagents. Each ImmPRESS Excel Amplified Staining Kit includes the following:

• BLOXALL^® Endogenous Enzyme Blocking Solution
• 2.5% Normal Horse Serum
• Amplifier Antibody (goat anti-rabbit IgG or goat anti-mouse IgG)
• ImmPRESS Excel Polymer Detection Reagent (horse anti-goat IgG)
• ImmPACT DAB EqV Substrate (Chromogen and Buffer)

Using the ImmPRESS Two-Step Amplified Polymer Systems

1. Add Primary Antibody

2. Add Amplifier Antibody

3 Add ImmPRESS Excel HRP Polymer Reagent

4 Add Enzyme Substrate

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