Immunohistochemistry Resource Guide
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Table of Contents
Immunohistochemistry Selection Guide
Avidin-Biotin Complex (ABC)-Based Detection
Choosing a VECTASTAIN® ABC Kit
> Customizing your VECTASTAIN® ABC Kit
ImmPRESS® One-Step Polymer Systems
> ImmPRESS® Two-Step Amplified Polymer Systems
> Choosing an ImmPRESS® Polymer Kit
> Multiple Antigen Labeling Simplified
M.O.M.® (Mouse on Mouse) Immunodetection Kits
> Counterstain/Substrate Compatibility Table
Counterstain/Substrate Compatibility Table
This table is designed as a reference to determine the optimal counterstain/substrate combination for your application. Considerations should be given to tissue type, antigen unmasking protocol and other detection parameters to achieve the desired staining intensity.
Substrate | Catalog Number | Vector® Hematoxylin and Hematoxylin | Vector® Methyl Green H-3402 | Vector® Nuclear Fast Red H-3403 |
|---|---|---|---|---|
ImmPACT® DAB EqV | Excellent Contrast | Excellent Contrast | Fair Contrast | |
ImmPACT® DAB (brown) | Excellent Contrast | Excellent Contrast | Fair Contrast | |
Vector® DAB (brown) | Excellent Contrast | Excellent Contrast | Fair Contrast | |
Vector® DAB-Ni (gray-black) | Excellent Contrast | Fair Contrast * | Good Contrast | |
ImmPACT® AEC (red) | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility | |
ImmPACT® AMEC Red (red) | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility | |
Vector® AEC (red) | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility | |
TMB (blue) | Color Incompatibility | Counterstain Incompatibility | Excellent Contrast | |
ImmPACT® VIP (purple) | Fair Contrast | Excellent Contrast | Poor Contrast | |
Vector® VIP (purple) | Fair Contrast | Excellent Contrast | Poor Contrast | |
ImmPACT® SG (blue-gray) | Poor Contrast | Good Contrast | Excellent Contrast | |
Vector® SG (blue-gray) | Poor Contrast | Good Contrast | Excellent Contrast | |
ImmPACT® NovaRED® (red) | Excellent Contrast | Excellent Contrast *** | Color Incompatibility | |
Vector® NovaRED® (red) | Excellent Contrast | Excellent Contrast *** | Color Incompatibility | |
ImmPACT® Vector® Red (magenta) | Excellent Contrast | Excellent Contrast | Color Incompatibility | |
Vector® Red (magenta) | Excellent Contrast | Excellent Contrast | Color Incompatibility | |
Vector® Black (black) | Excellent Contrast | Excellent Contrast * | Excellent Contrast | |
Vector® Blue (blue) | Color Incompatibility | Good Contrast | Excellent Contrast | |
BCIP/NBT (indigo) | Color Incompatibility | Excellent Contrast * | Excellent Contrast |
* This substrate shows a slight decrease in sensitivity following the methyl green protocol. This decrease can be minimized by reducing the heat incubation and acetone rinse times in the methyl green protocol.
** Substrate dissolves in acetone wash.
*** A slight color change in ImmPACT NovaRED and Vector NovaRED reaction product may be seen using methyl green.
Product | Mountants | Catalog Number |
|---|---|---|
Vector® Hematoxylin | Non-aqueous and Aqueous | |
Vector® Hematoxylin QS | Non-aqueous and Aqueous | |
Vector® Methyl Green | Non-aqueous | |
Vector® Nuclear Fast Red | Non-aqueous and Aqueous |
