Immunohistochemistry Resource Guide
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Table of Contents
Immunohistochemistry Selection Guide
Avidin-Biotin Complex (ABC)-Based Detection
Choosing a VECTASTAIN® ABC Kit
> Customizing your VECTASTAIN® ABC Kit
ImmPRESS® One-Step Polymer Systems
> ImmPRESS® Two-Step Amplified Polymer Systems
> Choosing an ImmPRESS® Polymer Kit
> Multiple Antigen Labeling Simplified
M.O.M.® (Mouse on Mouse) Immunodetection Kits
> Counterstain/Substrate Compatibility Table
ImmPRESS Polymer Detection Systems
ImmPRESS Polymer Reagents consist of unique micropolymers of highly active peroxidase or alkaline phosphatase enzyme attached to highly cross-adsorbed, affinitypurified secondary antibodies. This micropolymer conjugation technology allows a higher density of enzymes per antibody to bind to the target with minimal steric interference. The ImmPRESS Polymer Reagents produce outstanding immunohistochemistry and immunocytochemistry results due to increased target accessibility, binding specificity, and signal intensity along with low background staining.
- High sensitivity and very low background for crisp, strong staining
- Ready-to-use, one-step detection system – no mixing or titering
- Includes prediluted blocking serum
- Shorter assay time
- Non-biotin based
- Excellent resolution
- Especially suited for nuclear and membrane antigens
- Ideal for multiple antigen labeling
ImmPRESS VR Polymer Kits
ImmPRESS VR (Veterinary Reagents) Kits are additionally cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal models (bovine, goat, sheep, swine, horse, cat, dog, rabbit, rat, mouse).
Using the ImmPRESS Polymer Kits
1. Add Primary Antibody
2. Add ImmPRESS Reagent
3. Add Enzyme Substrate
