Purification, characterization, and identification of nucleic acids (DNA or RNA) often require analysis of the target in blotting applications. Nucleic acids are usually separated by size using gel electrophoresis and transferred to a membrane for detection using a complementary probe. Nucleic acid blotting applications require high specificity. The probes used go a long way toward … Continue reading Nucleic Acid Blotting Workflow

In Situ Hybridization Workflow Purification, characterization, and identification of nucleic acids (DNA or RNA) often require analysis of the target in blotting applications. Nucleic acids are usually separated by size using gel electrophoresis and transferred to a membrane for detection using a complementary probe. Nucleic acid blotting applications require high specificity. The probes used go … Continue reading In Situ Hybridization Workflow

Western blotting is a technique that is often used in the purification, characterization, and identification of proteins. Performing a western blot involves separating proteins by gel electrophoresis and transferring the separated proteins to a blotting membrane. Once blotted, protein can then be detected either directly (using a labeled primary antibody) or indirectly (using a primary … Continue reading Western Blotting Workflow

Reliable and Sensitive ABC Detection for IHC, IF, ELISA , and Blotting Applications Achieve sensitivity and specificity for your immunoassays with our time-tested, versatile Avidin-Biotin Complex (ABC) detection systems. Vector’s VECTASTAIN® Avidin-Biotin Complex Staining Kits (ABC Kits) exploit the high-affinity avidin-biotin interaction for reliable, highly sensitive detection of any biotinylated molecule. This method leverages avidin’s ultra-high … Continue reading ABC Kits

Amplified avidin-biotin detection kits for highly sensitive western blot detection Use the VECTASTAIN® ABC-AmP reagent to detect mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. This amplified ABC alkaline phosphatase (AP) reagent: Introduces more enzymes to the target site, providing signal amplification while maintaining low background Crates a signal that can be visualized using chemiluminescent, chemifluorescent, or … Continue reading ABC Kits For Western Blot Detection

Alkaline Phosphatase (AP) Substrates Vector offers alkaline phosphatase (AP) substrates in both original and ImmPACT® formulations. The original Vector®AP substrates offer a range of color choices and provide the following: Allow you to stain antigens in pigmented tissues and melanomas Enable localization of multiple antigens in a single tissue section (the combination of Vector® Red and Vector® Blue substrate, … Continue reading Alkaline Phosphatase (AP) Substrates

Amplified Fluorescent Staining Systems The VectaFluor™ Excel Amplified Fluorescent Staining System offers a convenient, non-biotin amplification method for immunofluorescence applications. Use the VectaFluor™ Excel kits to: Increase sensitivity by as much as three- to four-fold over that of a fluorophore-conjugated secondary antibody Enable unambiguous visualization of weakly expressed antigens Minimize dilution of potentially expensive primary … Continue reading Amplified Fluorescent Staining Systems

Anti-Avidin/Streptavidin Reagents Amplify avidin and streptavidin signals in a range of applications Use Anti-Avidin or Anti-Streptavidin amplification reagents to increase sensitivity in streptavidin or avidin/biotin detection systems. Anti-avidin does not bind streptavidin, and anti-streptavidin does not recognize avidin. Avidin or streptavidin amplification reagents can amplify signals in many applications, including: Immunohistochemistry In situ hybridization Microarray assays ELISAs Blots Vector offers … Continue reading Anti-Avidin/Streptavidin Reagents

Protein detection and visualization refer to the techniques used to identify, locate, and measure proteins in a sample. Antibodies are specialized proteins that bind specifically to target proteins (antigens), enabling their detection. These antibodies can be tagged with enzymes, fluorophores, or radioactive labels to produce a visible or measurable signal. Common methods utilizing antibodies include … Continue reading Antibodies

Antibodies to Tags & Labels Vector Labs offers you conjugated and unconjugated affinity-purified antibodies against several popular tags and labels used in blots, immunohistochemistry (IHC), in situ hybridization, and other applications. Antibodies are available against the following tags: Biotin DIG (digoxigenin) DNP (dinitrophenyl) Fluorescein

Unlock Epitope Accessibility for Optimal Immunostaining Formalin fixation is a standard method in immunohistochemistry (IHC) and immunofluorescence (IF) workflows to preserve tissue morphology. However, formalin induces cross-linking of proteins which can mask antigenic sites and hinder antibody binding. Antigen unmasking techniques restore epitope accessibility. The most common approach is heat-induced epitope retrieval (HIER) which involves … Continue reading Antigen Unmasking

Autofluorescence Quencher Reveal true immunofluorescence — even in the most challenging tissues In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background noise. Vector TrueVIEW® Autofluorescence Quencher Kit provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. Use TrueVIEW … Continue reading Autofluorescence Quencher

Avidin & Biotinylated Reagents Vector Laboratories was a pioneer in the field of histological staining. We were the first company to commercialize a Biotin-Avidin (now also Streptavidin) System, a substantial improvement over earlier staining methods. Many biotin molecules can be coupled to a protein, which allows the biotinylated protein to bind more than one molecule … Continue reading Avidin & Biotinylated Reagents

Bioconjugation Reagents SoluLINK® bioconjugation technology has been engineered to allow rapid and quantifiable conjugation of all classes of biomolecules. This technology is based on the reaction of HyNic with 4FB to form a stable bis-arylhydrazone bond. Unlike other conjugation chemistries, these linkers are chromophoric, meaning that they absorb light and can therefore be easily quantified with … Continue reading Bioconjugation Reagents

Glycans are carbohydrate chains linked to lipid or protein molecules through a process called glycosylation. Glycan modifications play critical roles in cell function in both healthy and diseased states from controlling proliferation to modulating the immune response. Lectins are part of a broader group called glycan-binding proteins that recognize glycan chains and mediate their functionality. … Continue reading Glycan Screening Kits