Alkaline Phosphatase (AP) Substrates

Vector offers alkaline phosphatase (AP) substrates in both original and ImmPACT^® formulations.

The original Vector^®AP substrates offer a range of color choices and provide the following:

• Allow you to stain antigens in pigmented tissues and melanomas
• Enable localization of multiple antigens in a single tissue section (the combination of Vector^® Red and Vector^® Blue substrate, for example, are useful in co-localization experiments)
• Vector^® Red and Vector^® Blue substrates can be viewed with both conventional light microscopy as well as fluorescence microscopy
• Vector^® AP substrates can be both non-aqueous and aqueously mounted with the exception of Vector^® Black, which should only be non-aqueously mounted
• Vector^® Blue substrate generates a product that can be cleared with citrus oil-based clearing agents and then permanently mounted
• The BCIP/NBT substrate is frequently used for nitrocellulose or in situ hybridization applications

ImmPACT^® Vector^® Red Substrate is the most sensitive red AP substrate:

• 2-4 fold greater sensitivity than the previous version
• Supplied with stock solutions in dropper bottles and optimized diluent
• Optimized specifically for immunohistochemistry applications
• Can be viewed with fluorescence as well as light microscopy

Enzyme Substrates / Microscopy Table

This table is designed as a reference to help you determine the optimal mounting medium for your application.

Additional Enzyme Substrate Characteristics

This table is designed as a reference to help you determine the optimal mounting medium for your application.