Amplified Fluorescent Staining Systems
The VectaFluor™ Excel Amplified Fluorescent Staining System offers a convenient, non-biotin amplification method for immunofluorescence applications. Use the VectaFluor™ Excel kits to:
- Increase sensitivity by as much as three- to four-fold over that of a fluorophore-conjugated secondary antibody
- Enable unambiguous visualization of weakly expressed antigens
- Minimize dilution of potentially expensive primary antibodies
This fluorescent staining system combines the following to amplify the fluorescence signal:
- Amplifier Antibody – a proprietary, high-affinity and cross-adsorbed Anti-Mouse IgG or Anti-Rabbit IgG antibody produced in goat
- VectaFluor™ DyLight® dye-conjugated Anti-Goat IgG antibody optimized for tight binding to the Amplifier Antibody
- Normal Horse Serum for blocking
The VectaFluor™ DyLight® dye-conjugated Horse Anti-Goat IgG is conjugated in a manner that ensures the maximum degree of labeling without compromising antibody affinity or specificity. DyLight® dyes offer advantages such as bright fluorescence staining, excellent photostability, and pH independence.
