Substrates play a crucial role in protein detection assays by enabling enzymatic reactions that generate colorimetric or chemiluminescent signals. These substrates are commonly used in immunohistochemistry (IHC), western blotting, and enzyme-linked immunosorbent assays (ELISA) to visualize target proteins with high sensitivity and specificity. Vector Laboratories provides high-quality substrate solutions.
- Alkaline Phosphatase (AP) Substrates – Designed for use with alkaline phosphatase (AP)-conjugated detection systems, these substrates produce distinct chromogenic or chemiluminescent signals for clear and reliable visualization in various immunoassays.
- DAB Enhancing Solution—Enhances the intensity and stability of 3,3′-Diaminobenzidine (DAB) substrate, a commonly used chromogen for horseradish peroxidase (HRP)-based detection, improving sensitivity and contrast in IHC and blotting applications.
- Horseradish Peroxidase (HRP) Substrates—Used in conjunction with HRP-conjugated antibodies, these substrates enable colorimetric or chemiluminescent detection, providing versatile options for high-contrast and high-sensitivity protein visualization.
By selecting the appropriate substrate, researchers can optimize signal clarity, enhance assay sensitivity, and achieve precise and reproducible protein detection results.
