What is Click Chemistry?

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Click Chemistry refers to a class of chemical reactions that are highly specific, fast, and efficient, allowing scientists to click two molecules together under simple conditions.

These reactions are broadly applicable across many areas of research, enabling precise labeling, modification, and conjugation of biomolecules. Unlike homobifunctional linkers, which can lead to random or multi-site binding, click chemistry reactions are directional, ensuring that molecules are conjugated at specific sites, preserving their orientation and functionality. Vector offers a comprehensive portfolio of high-quality click chemistry reagents to meet broad application needs.

Vector has a comprehensive
Click Chemistry Toolbox

providing solutions to meet your unique experimental requirements. Vector’s reagents are manufactured for exceptional quality, scalability, and reproducibility, with rigorous quality control ensuring dependable performance across a wide range of experimental applications.

Why Choose Click Chemistry?

Discover the unique advantages that make Click Chemistry an essential tool for bioconjugation reactions.

Property

Value

High Specificity

Highly specific reactions, even in complex biological environments

Rapid Reaction Kinetics

Fast, efficient reactions

High Yield and Purity

Near-quantitative yields with minimal by-products

Mild, Biocompatible Conditions

Compatibility with biological systems with gentle reaction conditions

Modularity

Modular components allow incorporation of diverse functional groups into molecules to meet application requirements

Scalable Reactions

Scalable reactions with reproducible results at both small and large scales

Solubility

Compatible with both aqueous and organic solvents enabling reactions in a wide range of experimental conditions.

Official Click Chemistry

TOOLBOX

By Vector Labs

Learn More

Choosing a Click Chemistry Solution:

Feature

CuAAC
Copper-Catalyzed Azide-­Alkyne Cy­cload­di­tion

SPAAC
Strain-Promoted Azide-­Alkyne Cy­cload­di­tion

Tetrazine-TCO Ligation

Reaction Partners

Azide and terminal alkyne

Azide and DBCO (cyclooctyne)

Tetrazine and trans-cyclooctene (TCO)

Reaction Kinetics

Fast

Moderate

Extremely fast

Catalyst Re­quire­ment

Requires a copper catalyst (Cu(I)). Accelerating copper-chelating ligands improve efficiency & cytotoxicity

No catalyst required (copper-free)

No catalyst required (copper-free)

Bio­com­pat­ibility

Limited

Highly bio­com­patible, ideal for in vivo applications

Highly bio­com­patible, ideal for in vivo applications

Cytotoxicity

Can be cytotoxic due to copper

Non-toxic (no copper catalyst)

Non-toxic (no copper catalyst)

Reaction Yield

High yield and efficiency in vitro

High yields but slower to reach completion than Tetrazine-TCO

High yields, very efficient

Reactant Con­cen­tra­tions

Typically requires micromolar Con­cen­tra­tions of both azide and alkyne, along with a copper catalyst, to achieve efficient yields.

Can function at micromolar to millimolar Con­cen­tra­tions, but lower Con­cen­tra­tions slow the reaction rate.

Highly efficient at nanomolar to micromolar Con­cen­tra­tions of both tetrazine and TCO.

Key Advantage

Well-established, efficient, and high-yielding in vitro

Copper-free, bioorthogonal and suitable with mild biological conditions

Fastest click reaction, bioorthogonal, ideal for applications requiring rapid kinetics

 

More Info about CuAAC

More Info about SPAAC

More Info about TCO/Tetrazine

Vector Click-&-Go® kits

Vector’s Click-&-Go® kits provide complete, ready-to-use solutions for researchers looking to simplify and accelerate their click chemistry experiments:

  • Click-&-Go® Enrichment Kits, allowing for the efficient capture of azide- or alkyne-labeled proteins using agarose resins for downstream mass spectrometry or proteomics.
  • Click-&-Go® Plus OPP Protein Synthesis Assay, enabling fast and sensitive detection of nascent proteins through fluorescence imaging.
  • Click-&-Go® IsoTaG Kits, empowering intact glycopeptide profiling on azide- or alkyne-modified glycopeptides
  • Click-&-Go® Fluorescent Imaging Kits, labeling alkyne-labeled molecules with fluorescent dyes.
  • Click-&-Go® Reaction Buffer Kits, performing click chemistry reactions in cell-based or biochemical assays.

Click chemistry offers high specificity, fast kinetics, and broad versatility, making it the ideal choice for scientists across drug development, biological research, materials science, and environmental sensing. With its variety of options, click chemistry enables safe and efficient reactions in living systems and synthetic environments alike. Vector’s comprehensive portfolio of click chemistry reagents provides researchers with reliable, high-quality solutions designed to streamline workflows and deliver consistent, reproducible results across a wide range of applications.

Click Chemistry Toolbox

A versatile collection of reagents, probes, and assay kits for biorthogonal labeling, imaging, enrichment, and proteomics.

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Get in touch with us at
customerservice@staging.vectorlabs.com
to take the next step in your bioconjugate journey.