Peroxidase (HRP) Substrates
Vector Laboratories offers two lines of Horseradish peroxidase (HRP) substrates.
Our original HRP substrate kits:
- Provide sensitivity greater than conventional substrates
- Are known for their consistency and reliability
- Supplied as concentrated stock solutions in convenient dropper bottles, stable for one year
The ImmPACT® line of HRP substrates offers the following advantages:
- 2-4 fold greater sensitivity than the original substrate kits
- Supplied with stock solutions in dropper bottles and optimized diluent
- Optimized specifically for immunohistochemistry applications
- Stock solutions stable for one year, working solution stable for up to 14 days at 4 ºC
Horseradish Peroxidase (HRP) Enzyme Substrates / Microscopy Table
This table is designed as a reference to help you determine the optimal mounting medium for your application.
Substrate | Catalog Number | Brightfield | Darkfield | Electron | Fluorescence | Spectral Imaging |
|---|---|---|---|---|---|---|
DAB (brown) | • | • | • |
| • | |
DAB-Ni (gray-black) | • | • | • |
| • | |
ImmPACT DAB (brown) | • | • | • |
| • | |
ImmPACT DAB EqV (brown) | • | • | • |
| • | |
Vector VIP (purple) | • | • | • |
| • | |
ImmPACT VIP (purple) | • | • | • |
| • | |
Vector SG (blue-gray) | • | • | • |
| • | |
ImmPACT SG (blue-gray) | • | • | • |
| • | |
Vector NovaRED (red) | • | • | • |
| • | |
ImmPACT NovaRED (red) | • | • | • |
| • | |
AEC (red) | • |
|
|
| • | |
ImmPACT AEC (red) | • |
|
|
| • | |
ImmPACT AMEC Red (red) | • |
|
|
| • | |
TMB (blue) | • |
|
|
| • |
Additional Horseradish Peroxidase (HRP) Enzyme Substrate Characteristics
This table is designed as a reference to help you determine the optimal mounting medium for your application.
Substrate | Catalog Number | Mounting Medium | Contrast in Pigmented Tissue | Multiple Labelling | Heat Resistant |
|---|---|---|---|---|---|
DAB (brown) | Non-aqueous |
| • | • | |
DAB-Ni (gray-black) | Non-aqueous |
| • |
| |
ImmPACT DAB (brown) | Non-aqueous |
| • | • | |
ImmPACT DAB EqV (brown) | Non-aqueous |
| • | • | |
Vector VIP (purple) | Non-aqueous | • | • |
| |
ImmPACT VIP (purple) | Non-aqueous | • | • |
| |
Vector SG (blue-gray) | Non-aqueous | • | • |
| |
ImmPACT SG (blue-gray) | Non-aqueous | • | • |
| |
Vector NovaRED (red) | Non-aqueous | • | • |
| |
ImmPACT NovaRED (red) | Non-aqueous | • | • |
| |
AEC (red) | Aqueous | • | • |
| |
ImmPACT AEC (red) | Aqueous | • | • |
| |
ImmPACT AMEC Red (red) | Aqueous | • | • |
| |
TMB (blue) | Non-Aqueous | • | • |
|
