Glysite Explorer isPLA Kit Comparison

	Glysite Explorer isPLA Kit	Mass Spec	Flow Cytometry	IHC
Sample requirement	1–2 mg	20 mg	<1 mg	1–2 mg
Sample cost (excluding primary antibody)	$25–50 / sample	High instrumentation or capital equipment fee	$60 / sample	$30–50 / sample
Output	Semi-quantitative	Quantitative / Qualitative	Quantitative	Semi-quantitative
Time-to-data	Same day	Days to weeks	1–3 days	Same day
High throughput screening	Medium	Low	Medium – High	High
Automation	Easy	Hard	Moderate	Easy
Spatial insights on protein glycosylation	Yes	No	No	No
Features	Spatial visualization of glycan-protein interactions High-throughput and same-day turnaround time Limited multiplexing – detect one target glycan and one target protein simultaneously	High sensitivity for identifying proteins and post-translational modifications Absolute quantification using selected workflow High cost and expertise barrier due to instrumentation and data processing Lack of spatial context	Multiplexing: detect numerous glycans and proteins in liquid samples Minimal sample needed per experiment (ideal for precious sample) Data interpretation requires expertise Lack of spatial organization	Multiplex profiling of proteins or glycans in cells and tissues Scalable and easy to automate Unable to probe glycan-protein interactions

	Glysite Explorer isPLA Kit	Mass Spec	Flow Cytometry	IHC
Sample requirement	1–2 mg	20 mg	<1 mg	1–2 mg
Sample cost (excluding primary antibody)	$25–50 / sample	High instrumentation or capital equipment fee	$60 / sample	$30–50 / sample
Output	Semi-Quantitative	Quantitative / Qualitative	Quantitative	Semi-Quantitative
Time-to-data	Same day	Days to weeks	1–3 days	Same day
High throughput screening	Medium	Low	Medium – High	High
Automation	Easy	Hard	Moderate	Easy
Spatial insights on protein glycosylation	Yes	No	No	No
Features	✓ Spatial visualization of glycan-protein interactions ✓ High-throughput and same-day turnaround time ✗ Limited multiplexing – detect one target glycan and one target protein simultaneously	✓ High sensitivity for identifying proteins and post-translational modifications ✓ Absolute quantification using selected workflow ✗ High cost and expertise barrier due to instrumentation and data processing ✗ Lack of spatial context	✓ Multiplexing: detect numerous glycans and proteins in liquid samples ✓ Minimal sample needed per experiment (ideal for precious sample) ✗ Data interpretation requires expertise ✗ Lack of spatial organization	✓ Multiplex profiling of proteins or glycans in cells and tissues ✓ Scalable and easy to automate ✗ Unable to probe glycan-protein interactions

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